Journal: EMBO Reports

Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

doi: 10.1038/s44319-024-00303-6

Figure Lengend Snippet: ( A ) Chromatin-bound levels of cohesin subunits and regulators assessed by Chromoflow flow cytometry. A representative experiment comparing A673 WT and STAG2 KO#1 is shown. Similar results were obtained comparing WT or Parental cells and STAG2 KO#1 or KO#2 in two independent experiments. ( B ) Immunoprecipitation of cohesin with anti-SMC1 from the indicated cell extracts followed by immunoblotting of cohesin and regulators. Colored dots below the panels identify each sample. ( C ) Quantification of the amount of the indicated proteins co-immunoprecipitated with anti-SMC1, relative to the amount of RAD21, in these four samples. Gray dots often overlap with black dots. .

Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

Techniques: Flow Cytometry, Immunoprecipitation, Western Blot

Journal: EMBO Reports

Article Title: STAG2 loss in Ewing sarcoma alters enhancer-promoter contacts dependent and independent of EWS::FLI1

doi: 10.1038/s44319-024-00303-6

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Mouse monoclonal anti-RAD21 clone 53A303; FC: 2 µg/ml , Merck , cat#05-908.

Techniques: Recombinant, Sequencing, Reverse Transcription, SYBR Green Assay, Software, Isolation